Junsen Biotechnology Co., Ltd
The best EGF supplier, Growth Factors supplier
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Product description :
Human fibronectin cell includes binding domain , heparin -binding domain and the CS1 site three functional areas. It consists of 574 amino acids with a molecular weight of 63kDa. It can greatly improve the efficiency of infection of mammalian cells, retroviral infection , and its principle is to : cell surface VLA-4 and VLA-5 , respectively, can be combined with the CS-1 domain site and a cell binding on Fibronectin , reverse viral vector may be combined with heparin binding domain . Fibronectin in the package container by the surface of culturing , the cells with the viral vector to the coexistence of high concentration , thereby enhancing the transfection efficiency of 30 to 70% . In addition Fibronectin can also stimulate lymphocytes to obtain the proliferation of thousands of times .
Product Features :
The retrovirus can be efficiently transferred to the gene of hematopoietic stem cells.
Avoid cytotoxicity due to the use of polybrene infection caused .
The same can stimulate the proliferation of lymphocytes obtained thousands of times the normal applicable infected cell lines .
You can get thousands of times to stimulate lymphocyte proliferation .
Appearance : Lyophilized Powder
Weight: 62,613 Dalton
Purity : SDS capillary electrophoresis > 97%
Coating : The Fibronectin with PBS buffer was diluted to a packing 20 ~ 100 μg / ml after the coated plates (non-tissue culture treated), the coating fluid 4 ~ 20 μg/cm2.
Precautions
1 Avoid repeated freezing and thawing .
2 When dissolved mix gently , do not use a vortex
Use: research use only and can not be used in clinical
Junsen Biotechnology Co., Ltd.
Contact: James Woo
E-mail: james@junsenbio.com